Abstract Background: The escalating challenge of antifungal resistance and the side effects associated with conventional drugs have intensified the search for natural alternatives. This study investigated the efficacy of Syzygium aromaticum (clove) extracts compared to standard antifungal agents against clinical oral Candida isolates from Libyan patients. Methods: Five Candida species (C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis) were isolated from patients in Gharyan City, Libya. The antifungal susceptibility to Amphotericin B, Miconazole and Nystatin was tested using the disc diffusion method. The activity of aqueous and alcoholic clove extracts (25%, 50%, 100%) and pure clove oil (Eugenol) was evaluated using a welldiffusion assay.

Background: The failure of spontaneous remyelination represents a critical therapeutic barrier in multiple sclerosis (MS) and other demyelinating pathologies. While genetic epidemiological studies have consistently linked Vitamin D Receptor (VDR) polymorphisms to disease susceptibility and severity, the specific cellular mechanisms translating these risk haplotypes into regenerative failure have remained unresolved. Methods: To deconstruct the demyelinated lesion microenvironment, we utilized high-resolution single-nucleus transcriptomics across distinct VDR genotypic cohorts (Risk vs. Wildtype). We integrated topological intercellular communication modelling to map shifts in local cellular crosstalk and employed computational pseudo time trajectory inference to evaluate the direct developmental consequences of genetic risk on the complete oligodendrocyte lineage. Results: Our analysis revealed a profound and targeted transcriptomic collapse of the RXRA heterodimer specifically within the resident microglial compartment of the VDR-Risk cohort. This intrinsic receptor uncoupling functionally neutralized the microglial neuroprotective state, resulting in a near-total cessation of Prosaposin (PSAP) secretion. Trajectory inference demonstrated that oligodendrocyte precursor cells (OPCs) deprived of this critical microglial PSAP-GPR37 trophic signalling axis suffered a severe developmental stall, structurally preventing their terminal maturation into functional, myelin-forming oligodendrocytes. Conclusions: These findings shift the pathophysiological paradigm of VDR genetic risk from generalized neuroinflammation to a highly targeted disruption of microglial oligodendroglia crosstalk. The RXRA-mediated PSAP-GPR37 signalling cascade is identified as a pivotal mechanistic vulnerability, offering a novel, targeted therapeutic pathway to overcome remyelination arrest.

Hypovitaminosis D is an escalating global health concern, yet high-power baseline mapping of its epidemiological burden across North Africa remains limited. Despite Libya’s abundant solar irradiance, localized studies suggest widespread vitamin D depletion. This study systematically pooled nationwide prevalence, clinical stratifications, temporal trends, and publication bias for vitamin D deficiency in Libya.A random-effects  systematic meta-analysis pooled  data  from  20  observational  studies  across  Western,  Eastern,  and Southern Libya, including 7,167 participants. Deficiency was defined as serum 25-hydroxyvitamin D [25(OH)D] <20 ng/mL. Between-study heterogeneity was explored using univariable and multivariable random-effects meta-regression. Publication bias was assessed with Egger’s regression, Begg’s rank correlation, and  trim-and-fill  analysis.The  pooled  national  prevalence  of  vitamin  D deficiency   was   71.07%   (95%   CI:   64.91%–76.81%),   with   substantial heterogeneity (I² = 96.2%; Cochran’s Q = 502.14; P < 0.0001). Pregnant women showed the highest subgroup prevalence at 80.02% (95% CI: 74.88%–84.31%), while the Southern desert region had the highest regional burden at 75.14% (95% CI: 71.05%–78.83%). Univariable meta-regression identified a significant temporal decline in prevalence (β = -0.041; P = 0.029). The multivariable model explained 24.15% ofbetween-study variance. Bias testing showed no significant funnel plot asymmetry. The findings confirm a Libyan “Mediterranean solar paradox,” where abundant sunlight does not ensure adequate vitamin D status. National prevention should prioritize food fortification, antenatal and pediatric supplementation, and standardized diagnostic assays.

Abstract Background Vitamin D is widely studied in multiple sclerosis (MS), yet its clinical relevance for disability and relapse activ ity remains uncertain, particularly in underrepresented populations. Objective To examine whether serum 25-hydroxyvitamin D [25(OH)D] levels are associated with disability or relapse activ ity in a multicenter cohort of Libyan adults with MS. Methods In a cross-sectional cohort of adults with MS (n = 369), the exposure was 25(OH)D scaled per 10 ng/mL (vitD10). Primary outcomes were the continuous Expanded Disability Status Scale (EDSS) and annualized relapse rate (ARR). We prespecified equivalence margins of ± 0.30 EDSS points and ± 0.25 ARR units, and fitted multivariable models adjusted for demographic, clinical, lifestyle, and treatment-related factors using HC3 robust standard errors. Two one-sided tests (TOST) were used to evaluate equivalence. Results For continuous EDSS, effect estimates were small and the 90% confidence interval lay within the ± 0.30 margin, supporting equivalence. Results for ARR were similarly within the prespecified ± 0.25 margin.There was little evidence of non-linearity, and sensitivity analyses, including measurement-error correction, did not materially change the findings. An exploratory threshold analysis at EDSS ≥ 4 suggested a small increase in odds (OR 1.47, 95% CI 1.01–2.15), which was not supported by continuous analyses and should be interpreted cautiously. Conclusions In this cross-sectional study, differences in 25(OH)D were unlikely to correspond to clinically meaningful dif ferences in disability. These findings suggest the absence of clinically meaningful effects on established disability, while not excluding potential roles in inflammatory activity or earlier disease processes. Ph

Abstract

Background: The "iron allergy hypothesis" suggests insufficient iron stores drive T-helper 2 polarisation and promote IgE sensitisation. We studied the association between serum ferritin and total IgE categories among Libyan children with recurrent wheeze. Methods: This cross-sectional study was conducted at Ali Omar Asker Hospital (Tripoli, Libya) from January 2023 to February 2026.Investigated 150 children aged 2-16 years diagnosed with recurrent wheezing. Serum ferritin and total IgE levels were classified as normal, low, or high based on laboratory ranges. Statistical analysis was performed using Pearson’s Chi-square, Fisher’s exact and Spearman’s rank correlation tests. Results: Among the 150 children, 23.8% had low ferritin, 76.2%were normal, and none were high. Overall, 54.7% had high IgE levels. The results showed IgE levels were nearly the same in both the normal and low ferritin groups (55.6% vs. 54.8%). This difference was not significant (χ² = 0.089, p = 0.912). Also, Spearman’s test confirmed no correlation between the two measures (rho = 0.012, p = 0.883).In subgroups, boys had a significantly higher rate of high IgE than girls (62.5% compared to 43.5%; p = 0.032). Regarding age, high IgE was found in 47.7% of children aged 2-6 years, 56.9% aged 7-11 years, and 58.5% aged 12-16 years. However, these variations were not statistically significant (p = 0.645). Finally, patient atopy and family history showed no significant links to ferritin or IgE levels.

Conclusion: Our study found no association between serum ferritin and total IgE levels in Libyan children with recurrent wheeze. However, the finding of male predominance in elevated IgE warrants more investigation. Further studies are needed to clarify nutrition-immunity interactions in pediatric respiratory diseases.

Toxoplasmosis is a worldwide contagious disease of humans and other warm-blooded animals, including birds. Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite responsible for Toxoplasmosis disease, which is clinically manifested chiefly in pregnant women and people with weakened immune systems. Clinical complications include abortion and stillbirths, encephalitis, pneumonia, brain and eye damage, and neonatal mortality. The genus Toxoplasma was first proposed in 1908 by Nicolle and Manceaux following the identification of asexual stages of similar parasites in the tissues of birds and mammals, and merozoites in the blood of North African rodents, Ctenodactylus gundi. At about the same time, Splendore independently described Toxoplasma in laboratory rabbits in Sao Paulo, Brazil, and Darling probably found it in man in Panama in the same year. Although several species were named, during the 1930s, it was shown that these were identical to the type species T. gondii. During the 1960, scientists provided evidence for the coccidian nature of the parasite. Then, between 1960 and 1970, the heterogeneous life cycle was elucidated by the discovery of sexual stages in the small intestine of cats, which followed the induction of infection in intermediate hosts by inoculation with cat feces. This review seeks to study the parasite and its responsibility to cause disease, condition prevalence, clinical manifestations accompanied by infection, and treatment protocols.

Nanotechnology is a modern technology and has many applications that include the manufacture of molecules or particles in the range of the nanoscale. Nanotechnology from the Greek word nano, meaning "dwarf". Nanoparticles are defined as single particles whose dimensions do not exceed 100 nanometers. The unique properties and features of nanoparticles are due to their small size, in addition to their chemical composition and surface structure. Different materials at the nanoscale lead to the development of new properties in industrial products, resulting in a real and impressive increase in industrial and medical applications. In this review, we will learn about this technology, its history, and the characteristics and shapes of nanoparticles. The focus will be on the application of nanotechnology in medicine, particularly in engineering living tissues with nanoscale scaffolds that simulate the functions of the extracellular matrix (ECM) to promote tissue recovery, replacement, and regeneration. It turns out that stem cells attached to a scaffold are more successful in adapting to their environment and performing the task of regeneration. The nerve endings in the body are attached to the scaffolding by weaving between the openings. This will cause them to act as a bridge to connect the cut sections. Over time, the scaffolds will dissolve and exit the body safely, leaving intact nerves in place. Some of the successfully used scaffolds are briefly identified, such as bone scaffolds, cardiac muscle scaffolds, and spinal cord engineering.

Nanotechnology is a modern technology and has many applications that include the manufacture of molecules or particles in the range of the nanoscale. Nanotechnology from the Greek word nano, meaning "dwarf". Nanoparticles are defined as single particles whose dimensions do not exceed 100 nanometers. The unique properties and features of nanoparticles are due to their small size, in addition to their chemical composition and surface structure. Different materials at the nanoscale lead to the development of new properties in industrial products, resulting in a real and impressive increase in industrial and medical applications. In this review, we will learn about this technology, its history, and the characteristics and shapes of nanoparticles. The focus will be on the application of nanotechnology in medicine, particularly in engineering living tissues with nanoscale scaffolds that simulate the functions of the extracellular matrix (ECM) to promote tissue recovery, replacement, and regeneration. It turns out that stem cells attached to a scaffold are more successful in adapting to their environment and performing the task of regeneration. The nerve endings in the body are attached to the scaffolding by weaving between the openings. This will cause them to act as a bridge to connect the cut sections. Over time, the scaffolds will dissolve and exit the body safely, leaving intact nerves in place. Some of the successfully used scaffolds are briefly identified, such as bone scaffolds, cardiac muscle scaffolds, and spinal cord engineering.

Toxoplasmosis is a worldwide contagious disease of humans and other warm-blooded animals, including birds. Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite responsible for Toxoplasmosis disease, which is clinically manifested chiefly in pregnant women and people with weakened immune systems. Clinical complications include abortion and stillbirths, encephalitis, pneumonia, brain and eye damage, and neonatal mortality. The genus Toxoplasma was first proposed in 1908 by Nicolle and Manceaux following the identification of asexual stages of similar parasites in the tissues of birds and mammals, and merozoites in the blood of North African rodents, Ctenodactylus gundi. At about the same time, Splendore independently described Toxoplasma in laboratory rabbits in Sao Paulo, Brazil, and Darling probably found it in man in Panama in the same year. Although several species were named, during the 1930s, it was shown that these were identical to the type species T. gondii. During the 1960, scientists provided evidence for the coccidian nature of the parasite. Then, between 1960 and 1970, the heterogeneous life cycle was elucidated by the discovery of sexual stages in the small intestine of cats, which followed the induction of infection in intermediate hosts by inoculation with cat feces. This review seeks to study the parasite and its responsibility to cause disease, condition prevalence, clinical manifestations accompanied by infection, and treatment protocols.

Background:

Vitamin D deficiency estimated to affect 50% of worldwide population of different civilizations and age groups.

Aim:

This retrospective cross-sectional study aimed to determine the prevalence and associated risk factors of vitamin D deficiency among residents of the Aljabal Algharbi region in Libya.

Methods:

A total of 657 laboratory test results were analyzed from patients in six cities: Gharian, Alasabaa, Alzintan, Jadu, Nalut, and Yafrin, covering the period from 2021 to 2024. Patient demographic data, including age and gender, were collected. According to clinical records, all tests were conducted using the Elecsys Vitamin D III Total kit for quantifying serum 25-hydroxyvitamin D [25(OH)D] levels. Vitamin D deficiency was defined as a serum 25(OH)D level of <30 ng/mL, while levels between 30–100 ng/mL were considered sufficient.

Results:

Overall, 506 patients (77%) were found to be vitamin D deficient. The prevalence was significantly higher in females (80%, 366/460) than in males (71%, 140/197) (p < 0.05). Among age groups, the highest deficiency rate (85%) was observed in individuals aged 31–50 years (p < 0.05), while the lowest was recorded in children aged 0-5 years. Multivariate analysis revealed that females (OR: 1.162, 95% CI: 1.015–1.330) and individuals aged 31–50 years (OR: 3.004, 95% CI: 1.197–7.537) were at significantly higher risk of deficiency.

Conclusion:

This study highlights a critical need for targeted public health interventions in the region. A coordinated effort involving healthcare providers, policymakers, and communities is essential to improve vitamin D status and mitigate the associated health risks.